An indirect competitive enzyme-linked immunosorbent assay (ELISA) for pentachlorophenol (PCP) was developed with a polyclonal antiserum produced against a hapten, 4-(2,3,5,6-tetrachloro-4-hydroxyphenoxy)-butyric acid, conjugated to bovine serum albumin (BSA). The ELISA of PCP showed
an IC50 of 18 ng/ml and a limit of detection (LOD) of 0.9 ng/ml at the optimal concentration of NaCl (0.2 M) in the assay buffer. This assay was slightly affected by up to 10% (v/v) methanol or acetone. The IC50 and Amax values showed little change in a pH range of 5 and 7. Very low cross
reactivities (≤2.5%) were observed for some structurally-related compounds, except for 2,3,4,6-tetrachlorophenol (5.6%) and 2,3,5,6-tetrachlorophenol (9.2%). The average recoveries of PCP from fortified water were in the range of 71-118%. The results by GC and ELISA for the same fortified
water samples showed non-significant difference (p>0.05). Finally, this ELISA was applied to monitor PCP in 100 river water samples and no PCP was detected. This ELISA is a good alternative tool for monitoring PCP residues in water samples.
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Document Type: Research Article
College of Resources and Environmental Sciences, China Agricultural University, Beijing, China
Department of Molecular Biosciences and Bioengineering, University of Hawaii, Honolulu, HI, USA
Department of Applied Chemistry, China Agricultural University, Beijing, China
September 1, 2007
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