The competitive enzyme immunoassays for detection of Campylobacter jejuni, C. coli and C. fetus subsp. fetus have been developed. Rabbit and hen immunoglobulins were prepared for these purposes. The working conditions of ELISAs, such as the concentrations of immunoreactants, incubation
temperatures and time, and the composition of the substrate have been established. The detection limits were in the range 5.0 104-3.2 106 cfu/ml. The application of chemiluminescent substrates did not result in any significant improvement of the assay's detectability and sensitivity. Prepared
antibodies showed rather high specificity and cross-reactivity profiles, and both rabbit and hen immunoglobulins were similar. Only IgY to C. jejuni cross-reacted with seven strains of C. jejuni and two other Campylobacter spp. A limited number of naturally and artificially contaminated food
samples were tested. The results obtained by means of an enzyme immunoassay were compared with those obtained from PCR or commercially available Singlepath® Campylobacter GLISA-Rapid Test. Poultry products were naturally contaminated with Campylobacters. The wild species were identified
as C. jejuni and C. coli.
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Document Type: Research Article
Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic
Department of Anthropology and Human Genetics, Charles University, Faculty of Science, Prague, Czech Republic
Veterinary and Pharmaceutical University Brno, Brno, Czech Republic
September 1, 2007
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