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Comparison of the determination of chloramphenicol residues in aquaculture tissues by time-resolved fluoroimmunoassay and with liquid chromatography and tandem mass spectrometry

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A competitive time-resolved fluoroimmunoassay (TR-FIA) was developed for the determination of chloramphenicol (CAP) residues in aquaculture tissues based on monoclonal antibodies. The limits of detection (LOD) were determined to be 0.04 ng g–1 and the limits of quantification (LOQ) were less than 0.15 ng g–1. The intra-assay variations were below 10% and the interassay variations ranged between 9.7 and 13.3%. The mean recoveries established at six concentration levels varied from 83.7–109.6% and the coefficient of variation was from 8.3–11.5%. The results obtained by the TR-FIA and ELISA showed a good correlation. The established TR-FIA was validated for the determination of incurred aquaculture tissues and confirmed by high-performance liquid chromatography and tandem mass spectrometry (LC-MS-MS). This proposed technique could be applied to routine residue analysis.

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Keywords: Chloramphenicol; ELISA; LC/MS-MS; residues; time-resolved fluoroimmunoassay

Document Type: Research Article

Affiliations: 1: Department of Chemical and Bioengineering, Huaiyin Institute of Technology, 223001, Huai'an, Jiangsu Province, China 2: School of Food Science and Technology, Southern Yangtze UniversityJiangsu Province, Wuxi, China

Publication date: September 1, 2006

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