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A comparison of time-resolved fluoroimmunoassay and ELISA in the detection of casein in foodstuffs

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With the aim of lowering the detection limit for casein in foods, three competitive assays are described: direct time-resolved fluoroimmunoassay (TR-FIA), using europium-conjugated antibody, indirect TR-FIA, using biotinylated antibody with europium-conjugated streptavidin and ELISA, using a HRP-conjugated secondary antibody. Food samples (instant potato, flour mix, packet soup, spice-mix) were analysed. Standard curve sensitivities in direct and indirect TR-FIAs did not differ significantly ( p =0.097), but both TR-FIAs were considerably less sensitive (both p −1 , respectively). The precision and working analyte range was similar in all three methods. Casein content measured in food products was comparable, using the three assays and rocket immunoelectrophoresis. The TR-FIA approach provided no improvement over the ELISA. All three assays allowed quantification of casein in foodstuffs in the order of 1–1.5 mg kg −1 , providing a basis for more rigorous validation and collaborative testing.
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Keywords: Casein; cow's milk allergy; enzyme-linked immunosorbent assay; food allergens; milk; time-resolved fluoroimmunoassay

Document Type: Research Article

Affiliations: National Veterinary Institute, Oslo, Norway

Publication date: September 1, 2005

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