@article {Sethi:2005:0954-0105:59,
title = "Immunological characterization of Karnal bunt resistance by differential response of pathogen restriction in a dual culture system",
journal = "Food and Agricultural Immunology",
parent_itemid = "infobike://tandf/cfai",
publishercode ="tandf",
year = "2005",
volume = "16",
number = "1",
publication date ="2005-03-01T00:00:00",
pages = "59-71",
itemtype = "ARTICLE",
issn = "0954-0105",
eissn = "1465-3443",
url = "https://www.ingentaconnect.com/content/tandf/cfai/2005/00000016/00000001/art00006",
doi = "doi:10.1080/09540100400016024",
keyword = "dual culture, Karnal bunt, mycelial biomass, teliospores, immunoassays, histochemical staining",
author = "Sethi, N. and Garg, G. K. and Singh, U. S. and Jaiswal, J. P. and Kumar, A.",
abstract = "For monitoring disease progression and evaluating Karnal bunt resistance dual cultures were established on callus induced from mature embryos of eight host varieties and two non-host varieties inoculated with a sporidial suspension of a virulent strain of Tilletia indica pathogen. Histochemical studies of dual-cultured calluses showed fungal mycelial growth and formation of chlamydospore-like structures that were more profuse in susceptible lines than in resistant and non-host lines. The formation of a zone of hollowness, i.e. a gap between mycelia and host tissue, an indication of pathogen restriction, was seen in resistant hosts and non-hosts but not in susceptible hosts. Detection and quantification of mycelia and teliospores in dual-cultured calluses by immunoassays revealed remarkable difference in the deposition of mycelia and teliospores-like structures. Susceptible calluses supported more abundant mycelial growth than the resistant and non-host co-cultured calluses based on the binding reaction of polyclonal antibodies.",
}