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Immunological characterization of Karnal bunt resistance by differential response of pathogen restriction in a dual culture system

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For monitoring disease progression and evaluating Karnal bunt resistance dual cultures were established on callus induced from mature embryos of eight host varieties and two non-host varieties inoculated with a sporidial suspension of a virulent strain of Tilletia indica pathogen. Histochemical studies of dual-cultured calluses showed fungal mycelial growth and formation of chlamydospore-like structures that were more profuse in susceptible lines than in resistant and non-host lines. The formation of a zone of hollowness, i.e. a gap between mycelia and host tissue, an indication of pathogen restriction, was seen in resistant hosts and non-hosts but not in susceptible hosts. Detection and quantification of mycelia and teliospores in dual-cultured calluses by immunoassays revealed remarkable difference in the deposition of mycelia and teliospores-like structures. Susceptible calluses supported more abundant mycelial growth than the resistant and non-host co-cultured calluses based on the binding reaction of polyclonal antibodies.
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Keywords: Karnal bunt; dual culture; histochemical staining; immunoassays; mycelial biomass; teliospores

Document Type: Research Article

Affiliations: 1: Department of Molecular Biology & Genetic Engineering, College of Basic Sciences and HumanitiesG. B. Pant University of Agriculture and Technology, Pantnagar, -263145, India 2: Department of Plant Pathology, G. B. Pant University of Agriculture and Technology, Pantnagar, -263145, India 3: Department of Genetics & Plant Breeding, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar, -263145, India

Publication date: March 1, 2005

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