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Monoclonal antibodies for fumonisins B 1 , B 2 and B 3

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There has been only one report of fumonisin antibodies produced from a fumonisin-bovine serum albumin (BSA) immunogen in the past. Other, more exoteric, carriers, such as cholera toxin and keyhole limpet hemocyanin, have also been used. The resulting antibodies have a wide range of sensitivity and selectivity for the most common fumonisins B 1 , B 2 and B 3 . We report on fumonisin monoclonal antibodies produced in typical fashion from an immunogen consisting of FB 1 conjugated to BSA through its terminal amino group. These antibodies have excellent cross-reactivity with the three main fumonisins: FB 1 , 100%, FB 2 , 94%, and FB 3 , 72%, adequate sensitivity with an IC 50 of 430 ng/mL for FB 1 , and show excellent correlation between ELISA and total fumonisins as determined by HPLC, with a slope of 0.985 and an r 2 of 0.987.
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Keywords: ELISA; Fumonisin; antibodies; monoclonal

Document Type: Research Article

Publication date: June 1, 2003

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