@article {Irwin:2003:0954-0105:93,
title = "Development of an enzyme-linked immunosorbent assay for the detection and quantification of the insecticide tebufenozide in wine",
journal = "Food and Agricultural Immunology",
parent_itemid = "infobike://tandf/cfai",
publishercode ="tandf",
year = "2003",
volume = "15",
number = "2",
publication date ="2003-06-01T00:00:00",
pages = "93-104",
itemtype = "ARTICLE",
issn = "0954-0105",
eissn = "1465-3443",
url = "https://www.ingentaconnect.com/content/tandf/cfai/2003/00000015/00000002/art00003",
doi = "doi:10.1080/09540100400003188",
keyword = "ELISA, Tebufenozide, protein conjugates, polyclonal antibodies, wine, pesticide, immunoassay",
author = "Irwin, Jennifer A. and Tolhurst, Rachel and Jackson, Phillip and Gale, Kevin R.",
abstract = "A CD-ELISA assay has been developed for the quantification of the insecticide tebufenozide in red and white wine. Polyclonal antisera, raised against both KLH- and OVA-linked immunogens of the hapten 1-tert-butyl-2-[4-(carboxypropan-3-yl)benzoyl]-1-(3,5-dimethyl benzoyl)hydrazide NHS ester ( Ib ) were trialled for use in a quantitative immunoassay and both were found to be suitable for use, with IC 50 values in the vicinity of 10 g L 1 regardless of the carrier protein used and matrix (red versus white wine). The limits of detection were 0.7 g L 1 and 3 g L 1 for OVA- and KLH-linked polyclonal sera, respectively. In both cases, the IgG fraction isolated from the antisera was highly specific for tebufenozide and its hapten derivatives, showing no detectable cross-reactivity with eleven other potential pesticide contaminants used in the wine-making industry. Spiked red and white wine samples were successfully analyzed over the range 10-1000 g L 1 using the immunoassay developed with IgG isolated from the antiserum rabbit anti-OVA- Ib as the immobilized capture antibody. Reported maximum residue limits for wine are 100 g L 1 and thus would be readily detected using this technique. The immunoassay format represents a cost-effective, relatively simple means of screening large numbers of samples for the presence of this insecticide.",
}