Skip to main content
padlock icon - secure page this page is secure

Development of an enzyme-linked immunosorbent assay for the detection and quantification of the insecticide tebufenozide in wine

Buy Article:

$63.00 + tax (Refund Policy)

A CD-ELISA assay has been developed for the quantification of the insecticide tebufenozide in red and white wine. Polyclonal antisera, raised against both KLH- and OVA-linked immunogens of the hapten 1-tert-butyl-2-[4-(carboxypropan-3-yl)benzoyl]-1-(3,5-dimethyl benzoyl)hydrazide NHS ester ( Ib ) were trialled for use in a quantitative immunoassay and both were found to be suitable for use, with IC 50 values in the vicinity of 10 g L −1 regardless of the carrier protein used and matrix (red versus white wine). The limits of detection were 0.7 g L −1 and 3 g L −1 for OVA- and KLH-linked polyclonal sera, respectively. In both cases, the IgG fraction isolated from the antisera was highly specific for tebufenozide and its hapten derivatives, showing no detectable cross-reactivity with eleven other potential pesticide contaminants used in the wine-making industry. Spiked red and white wine samples were successfully analyzed over the range 10-1000 g L −1 using the immunoassay developed with IgG isolated from the antiserum rabbit anti-OVA- Ib as the immobilized capture antibody. Reported maximum residue limits for wine are 100 g L −1 and thus would be readily detected using this technique. The immunoassay format represents a cost-effective, relatively simple means of screening large numbers of samples for the presence of this insecticide.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: ELISA; Tebufenozide; immunoassay; pesticide; polyclonal antibodies; protein conjugates; wine

Document Type: Research Article

Publication date: June 1, 2003

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more