Skip to main content
padlock icon - secure page this page is secure

Development of ELISA and Sensor-based Assays for the Detection of Ethynyl Estradiol in Bile

Buy Article:

$63.00 + tax (Refund Policy)

A polyclonal antibody, EEAb1, was produced against the synthetic steroid hormone, ethynyl estradiol (EE). EEAb1 demonstrated low levels of cross-reactivity with related compounds. Competitive and inhibitive ELISA assays were developed along with a biosensor-based assay using a BIAcoreĀ® 3000 for the detection of EE. For EEAb1 the detection ranges were 6-100 000 ng ml−1 in the competitive ELISA, 3-50 000 ng ml−1 in the inhibitive ELISA and 12-100 000 ng ml−1 in the biosensor-based assay. The competitive ELISA data corresponded more closely to the 4-parameter curve model used and also required less time to perform than the inhibitive ELISA. The assays were optimized for use with bile. The bile was diluted 1/2 with buffer (phosphate-buffered salin or Hepes-buffered saline) prior to analysis. The assay formats described in this paper offer quick and simple methods for the detection of ethynyl estradiol in bile samples from cattle.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: ELISA; Ethynyl estradiol; bile; biosensor; polyclonal antibody

Document Type: Research Article

Affiliations: School of Biotechnology Dublin City University Dublin 9

Publication date: March 1, 2003

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more