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Monoclonal Antibody to Fenbendazole: Utility in Residue Studies

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A monoclonal antibody-based ELISA was developed for fenbendazole, a widely used benzimidazole anthelmintic, with approved uses in cattle and other food animals. The antibody was elicited using as hapten 2-succinamido-5(6)-phenylthiobenzimidazole, which was conjugated with bovine serum albumin to produce an immunogen and with horseradish peroxidase to prepare a labeled ligand. The ELISA was performed on aqueous extracts of bovine liver or diluted milk samples. In bovine liver, the limit of detection was 200 ppb; in the milk matrix, the limit of detection was 3 ppb. The ELISA method is a simple approach to screen food samples for residues of fenbendazole.
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Keywords: Fenbendazole; benzimidazole anthelmintic; drug residue; monoclonal antibody

Document Type: Research Article

Affiliations: USDA Agricultural Research Service, Western Regional Research Center, 800 Buchanan Street, Albany CA 94710, USA

Publication date: December 1, 2002

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