this page is secure
Production and Characterization of Murine Single Chain Fv Antibodies to Aflatoxin B1 Derived From a Pre-immunized Antibody Phage Display Library System
The production and characterization of recombinant antibodies to aflatoxin B1 (AFB1), a potent mycotoxin and carcinogen is described. The antibody fragments produced were then applied for use in a surface plasmon resonance-based biosensor (BIAcore), which measures biomolecular interactions in 'real-time'. Single chain Fv (scFv) antibodies were generated to aflatoxin B1 from an established phage display system, which incorporated a range of different plasmids for efficient scFv expression. The scFv's were used in the development of a competitive ELISA, and also for the development of surface plasmon resonance (SPR)-based inhibition immunoassays. They were found to be suitable for the detection of AFB1, in this format, with the assays being sensitive and reproducible.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics
Keywords: Aflatoxin B1; phage display technology; single chain Fv; surface plasmon resonance
Document Type: Research Article
Affiliations: 1: School of Biotechnology, Dublin City University, Dublin 9, Ireland 2: National Centre for Sensor Research, Dublin City University, Dublin 9, Ireland
Publication date: December 1, 2002
- Editorial Board
- Information for Authors
- Subscribe to this Title
- Ingenta Connect is not responsible for the content or availability of external websites
Receive new issue alert