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Production and Characterization of Murine Single Chain Fv Antibodies to Aflatoxin B1 Derived From a Pre-immunized Antibody Phage Display Library System

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The production and characterization of recombinant antibodies to aflatoxin B1 (AFB1), a potent mycotoxin and carcinogen is described. The antibody fragments produced were then applied for use in a surface plasmon resonance-based biosensor (BIAcore), which measures biomolecular interactions in 'real-time'. Single chain Fv (scFv) antibodies were generated to aflatoxin B1 from an established phage display system, which incorporated a range of different plasmids for efficient scFv expression. The scFv's were used in the development of a competitive ELISA, and also for the development of surface plasmon resonance (SPR)-based inhibition immunoassays. They were found to be suitable for the detection of AFB1, in this format, with the assays being sensitive and reproducible.
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Keywords: Aflatoxin B1; phage display technology; single chain Fv; surface plasmon resonance

Document Type: Research Article

Affiliations: 1: School of Biotechnology, Dublin City University, Dublin 9, Ireland 2: National Centre for Sensor Research, Dublin City University, Dublin 9, Ireland

Publication date: December 1, 2002

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