@article {Asis:2002:0954-0105:201,
title = "Determination of Aflatoxin B1 in Highly Contaminated Peanut Samples Using HPLC and ELISA",
journal = "Food and Agricultural Immunology",
parent_itemid = "infobike://tandf/cfai",
publishercode ="tandf",
year = "2002",
volume = "14",
number = "3",
publication date ="2002-09-01T00:00:00",
pages = "201-208",
itemtype = "ARTICLE",
issn = "0954-0105",
eissn = "1465-3443",
url = "https://www.ingentaconnect.com/content/tandf/cfai/2002/00000014/00000003/art00004",
doi = "doi:10.1080/09540100220145000",
keyword = "CONTAMINATION LIMITS, AFLATOXIN ANALYSIS, AFLATOXINS, PEANUTS, ELISA VALIDATION",
author = "Asis, Ram{\’o}n and Di Paola, Romina D. and Aldao, Mario A. J.",
abstract = "The precision, accuracy, detection limit and peanut matrix influence of an ELISA were analysed in the determination of aflatoxin B1. The assay was performed on two different reference samples: peanut extract and peanut paste, that were spiked with known amounts of aflatoxins. The lower detectable level was 0.5 g kg-1. The average intra-assay precision expressed as coefficient of variation (CV) was 11.7% for concentrations between 2.54 and 901 g kg-1 and the average inter-assay precision was 29.7% for the same range of concentrations. The average accuracy measured by a recovery assay in samples that contained only aflatoxin B1 was 107%. The correlation between the ELISA and high-performance liquid chromatography (HPLC) applied to 28 peanut samples artificially contaminated with Aspergillus flavus and A. parasiticus spores showed a high correlation (r = 0.977, P < 0.0001). The detection limits and matrix influence associated with our ELISA procedure were more sensitive than those reported for other ELISA procedures due to specific antiserum treatment.",
}