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Analysis of -Lactam Antibiotics Using a Microbial Receptor Protein-based Biosensor Assay

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An optical biosensor assay for detection of -lactam antibiotics in milk based on a microbial receptor protein was developed. The assay uses a general sensor surface previously described with a small organic molecule (H1) immobilized. A conjugate between a -lactam (cephalosporin C) and a monoclonal H1 antibody is injected across the sensor surface before injection of the sample mixed with receptor protein. Receptor inhibited by -lactam residues in the milk sample will not bind to the sensor surface and the reduction in response is inversely related to the -lactam concentration of the sample. The detection limit for a number of commonly used -lactams was below or near the respective maximum residue limit and the relative standard deviation (CV) for penicillin G in milk was 6-12% in the interval 2.0-12.5  g kg-1. For application in the field further optimization is needed to solve problems related to non-specific binding to the sensor surface.
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Keywords: ANTIBIOTICS; ASSAY; BETA; BIOSENSOR; MILK; PROTEIN; RECEPTOR

Document Type: Research Article

Publication date: June 1, 2002

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