Skip to main content
padlock icon - secure page this page is secure

Analysis of -Lactam Antibiotics Using a Microbial Receptor Protein-based Biosensor Assay

Buy Article:

$63.00 + tax (Refund Policy)

An optical biosensor assay for detection of -lactam antibiotics in milk based on a microbial receptor protein was developed. The assay uses a general sensor surface previously described with a small organic molecule (H1) immobilized. A conjugate between a -lactam (cephalosporin C) and a monoclonal H1 antibody is injected across the sensor surface before injection of the sample mixed with receptor protein. Receptor inhibited by -lactam residues in the milk sample will not bind to the sensor surface and the reduction in response is inversely related to the -lactam concentration of the sample. The detection limit for a number of commonly used -lactams was below or near the respective maximum residue limit and the relative standard deviation (CV) for penicillin G in milk was 6-12% in the interval 2.0-12.5  g kg-1. For application in the field further optimization is needed to solve problems related to non-specific binding to the sensor surface.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics


Document Type: Research Article

Publication date: June 1, 2002

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more