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Immunoassay and HPLC Detection of Halofuginone in Chicken Liver Samples Obtained from Commercial Slaughterhouses: A Combined Study

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Halofuginone (Hal) is a feed additive used worldwide to prevent coccidiosis in commercial poultry production. The current regulatory method for determining the action level of Hal residues in poultry involves measuring parent Hal in liver tissue by HPLC. A competitive enzyme-linked immunosorbent assay (cELISA) for Hal was evaluated with respect to HPLC in determining Hal in 473 samples of chicken liver tissue obtained from commercial poultry slaughterhouses. Chicken liver samples were divided, and analyzed by both the US Department of Agriculture, Food Safety and Inspection Service's(FSIS's) regulatory method, and by the US Department of Agriculture, Agricultural Research Service's (ARS's) cELISA method described here. The lower level of detection for Hal was 50 ppb by the FSIS HPLC method and 38 ppb by the ARS cELISA method. The lower cutoff limit for this study was 50 ppb as mandated by FSIS SOP. There was good agreement in the results obtained by HPLC and cELISA. In addition, the cELISA method does not require the use of organic solvents. These data clearly demonstrate that the cELISA method could be used as a screening method for the analysis of Hal in chicken liver tissue. If the cELISA had been used as a screening tool in this study, then only six samples (≥ 100 and < 160 ppb) out of the 473 samples analyzed would have required further analysis by HPLC. The organic solvent waste (over 100 l) generated by the HPLC method would have then been reduced to approximately 1.272 l, a considerable time and cost savings in waste management.
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Document Type: Research Article

Publication date: March 1, 2002

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