Detection of Photochemically Induced Cuticle-Bound Residues of Parathion by Immunoassay
An indirect competitive ELISA was developed using a polyclonal rabbit antiserum against diethyl thionophosphates. It was found to show its highest sensitivity for the organophosphorus insecticide parathion. This assay was applied to detect bound residues of parathion formed by photodegradation in fruit cuticles. Detection of bound residues was successful in the dot-blot immunoassay as well as in the ELISA test format. In accordance to earlier model studies on the photochemistry of parathion, the same results were achieved using cuticles treated with the photoproduct nitrosoparathion.
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Document Type: Research Article
Publication date: March 1, 2002