Comparison of an ELISA and a HPLC for Determination of Ciprofloxacin Residues in Pork
To determine ciprofloxacin (CPFX) residues in pork, an ELISA and a high-performance liquid chromatography (HPLC) procedure were developed and compared on sensitivity, precision and accuracy. For ELISA, CPFX was converted by an active ester method into CFPX-human serum albumin (CPFX-HSA) and CFPX-bovine serum albumin (CPFX-BSA), and the latter allowed the production of CPFX-specific rabbit antisera for the development of an indirect competitive ELISA procedure. For HPLC, an ODS reverse phase column was used with ultraviolet detection, and triethylamine and acetonitrile (87:13) were employed as mobile phase. The limits of detection (LODs) were 0.32 ng ml-1 for ELISA and 10 ng ml-1 for HPLC. The linear detection ranges were 0.32-5000 ng ml-1 for ELISA and 10-5120 ng ml-1 for HPLC. The coefficients of variation were 4.51-11.50% for ELISA and 0.70-9.10% for HPLC over the range of CPFX concentrations of each method. The mean recovery of HPLC (80.58%) was higher than that of ELISA (66.18%). The results suggested that the ELISA, with its high detection throughput and excellent sensitivity and specificity, could be used as a screening method for CPFX residues in pork. The HPLC, for its great precision and accuracy, was an effective confirmatory method for CPFX residues in pork.
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Document Type: Short Communication
Publication date: September 1, 2001