Skip to main content
padlock icon - secure page this page is secure

Highly Sensitive Enzyme Immunoassays for the Detection of -Lactam Antibiotics

Buy Article:

$63.00 + tax (Refund Policy)

Specific and sensitive antibodies against -lactam antibiotics are difficult to raise due to the chemical reactivity of the -lactam ring. The antibiotic-protein conjugates used as the immunogen can easily react with primary amino groups as for instance the -amino group of lysine side chains in proteins. This leads to the degradation of the immunogen to a complex mixture and, therefore, to an unpredictable immune response of the host animal. We produced antisera against the hydrolyzed form of -lactam antibiotics by immunizing rabbits with stable conjugates mimicking this form. Addition of penicillinase in the immunoassay leads to hydrolysis of the -lactam antibiotic, which is recognized by the antiserum. In a competitive enzyme-immunoassay, benzylpenicillin could be detected at levels of 0.05 ng ml-1 and cloxacillin at levels of 0.1 ng ml-1 in pasteurized milk. The sensitivity and also the selectivity of these EIAs are remarkably high and offer a wide range of different applications. The procedure should be applicable for other -lactam antibiotics, and may therefore in future play an important role in food quality control and assurance.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics


Document Type: Research Article

Publication date: September 1, 2001

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more