@article {Driedger:2001:0954-0105:33,
title = "Development of an Antibody against Diosgenin and Spiroaminoketal Alkaloids",
journal = "Food and Agricultural Immunology",
parent_itemid = "infobike://tandf/cfai",
publishercode ="tandf",
year = "2001",
volume = "13",
number = "1",
publication date ="2001-03-01T00:00:00",
pages = "33-38",
itemtype = "ARTICLE",
issn = "0954-0105",
eissn = "1465-3443",
url = "https://www.ingentaconnect.com/content/tandf/cfai/2001/00000013/00000001/art00003",
doi = "doi:10.1080/09540100051074185",
keyword = "DIOSGENIN, ELISA, SOLASODINE, WILD, SOLANINE, TOMATINE, ANTIBODY",
author = "Driedger, Darcy R. and Sporns, Peter",
abstract = "An antibody was developed that exhibited affinity for diosgenin as well as the spiroaminoketal alkaloids solasodine and tomatidine. Rabbits were immunized with a diosgenin conjugate prepared by succinylation of diosgenin, followed by purification with a silica solid-phase extraction cartridge and active ester conjugation to Limulus polyphemus hemocyanin (LPH). Rabbit serum was evaluated using an indirect competitive ELISA with diosgenin-BSA as the coating conjugate. A reduction in absorbance of 50% could be obtained using concentrations of ca. 0.8 M diosgenin, 30M solasodine and 100M tomatine. The antibody exhibited no affinity for solanidine, a non-spiral glycoalkaloid.",
}