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Development of an Antibody against Diosgenin and Spiroaminoketal Alkaloids

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An antibody was developed that exhibited affinity for diosgenin as well as the spiroaminoketal alkaloids solasodine and tomatidine. Rabbits were immunized with a diosgenin conjugate prepared by succinylation of diosgenin, followed by purification with a silica solid-phase extraction cartridge and active ester conjugation to Limulus polyphemus hemocyanin (LPH). Rabbit serum was evaluated using an indirect competitive ELISA with diosgenin-BSA as the coating conjugate. A reduction in absorbance of 50% could be obtained using concentrations of ca. 0.8  M diosgenin, 30M solasodine and 100M tomatine. The antibody exhibited no affinity for solanidine, a non-spiral glycoalkaloid.
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Keywords: ANTIBODY; DIOSGENIN; ELISA; SOLANINE; SOLASODINE; TOMATINE; WILD

Document Type: Research Article

Affiliations: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada

Publication date: March 1, 2001

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