Skip to main content
padlock icon - secure page this page is secure

Immunochemical Approaches to the Detection of Sulfathiazole in Animal Tissues

Buy Article:

$63.00 + tax (Refund Policy)

A series of ELISAs based on anti-sulfathiazole (STZ) monoclonal antibodies (MAbs) were developed that can detect STZ below the tolerance level in tissues. The IC50 values (concentrations of STZ inhibiting 50% of binding in a competitive ELISA) for each MAb ranged from 6 to 21 ng ml-1 of STZ. Immunoassays based upon two MAbs, STZ-23 and SDM44, were evaluated for their assay performance using swine liver samples spiked with STZ. Swine liver tissue was analyzed without a complex sample preparation step, with only a simple dilution of swine liver extract being necessary. A good correlation between immunoassay results and spike levels in swine liver (R2 >0.98) was obtained, demonstrating that the immunoassays are capable of quantifying STZ in swine liver. The cross-reactivities of other sulfonamide drugs and STZ metabolites in these immunoassays were also examined.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: ANTIBODY; FOOD; IMMUNOASSAY; MONOCLONAL; SAFETY; SULFATHIAZOLE

Document Type: Research Article

Affiliations: 1: Food Animal Protection Research Laboratory, Agricultural Research Service, US Department of Agriculture, College Station, TX 77845, USA 2: L. J. L. Biosystems, 404 Pasman Drive, Sunnyvale, CA 94089, USA

Publication date: March 1, 2001

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more