A series of ELISAs based on anti-sulfathiazole (STZ) monoclonal antibodies (MAbs) were developed that can detect STZ below the tolerance level in tissues. The IC50 values (concentrations of STZ inhibiting 50% of binding in a competitive ELISA) for each MAb ranged from 6 to 21 ng ml-1 of STZ. Immunoassays based upon two MAbs, STZ-23 and SDM44, were evaluated for their assay performance using swine liver samples spiked with STZ. Swine liver tissue was analyzed without a complex sample preparation step, with only a simple dilution of swine liver extract being necessary. A good correlation between immunoassay results and spike levels in swine liver (R2 >0.98) was obtained, demonstrating that the immunoassays are capable of quantifying STZ in swine liver. The cross-reactivities of other sulfonamide drugs and STZ metabolites in these immunoassays were also examined.
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Document Type: Research Article
Food Animal Protection Research Laboratory, Agricultural Research Service, US Department of Agriculture, College Station, TX 77845, USA
L. J. L. Biosystems, 404 Pasman Drive, Sunnyvale, CA 94089, USA
March 1, 2001
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