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A New Monoclonal Antibody for the Sensitive Detection of Cyanazine and Other s-Triazines in Water by ELISA

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A new monoclonal antibody with specificity for s-triazine herbicides was prepared using a tertbutyl-s-triazine hapten. An assay was developed, employing covalently immobilized hapten and monoclonal antibody labeled with horseradish peroxidase. This assay system had a detection limit of 0.01 gl-1 cyanazine, with a coefficient of variation less than 3%. Analysis on three different spiked water types showed an average recovery of 101% at 0.1 gl-1 cyanazine. Cross-reactions were found for atrazine, terbuthylazine and propazine in the range 18-62%. This immunoassay is therefore capable of detecting four different s-triazine herbicides at concentrations well below the limit value for drinking water of 0.1 gl-1 stipulated by the EEC Council Directive on drinking water.
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Keywords: COVALENT IMMOBILIZATION; CYANAZINE; HERBICIDE; IMMUNOASSAY; MONOCLONAL ANTIBODY; PESTICIDE

Document Type: Research Article

Affiliations: 1: Department of Geochemistry, Geological Survey of Denmark and Greenland, Thoravej 8, DK-2400 Copenhagen NV, Denmark 2: Department of Applied Immunology, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen S, Denmark 3: Exiqon A/S, Bygstubben 9, DK-2950 Vedbœ:;k, Denmark

Publication date: December 1, 2000

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