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Generation of an Anti-idiotypic Antibody as a Surrogate Ligand for Sulfamethazine in Immunoassay Procedures

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We report the generation of an anti-idiotypic antibody, clone 12E12, against antisulfamethazine (SMZ) as a surrogate ligand for SMZ in immunoassay procedures. This has been achieved by using the primary monoclonal anti-SMZ antibody as an immunogen in hybridoma technology followed by screening of the resulting hybridromas for binding to the SMZ site of the primary anti-SMZ antibody. Characterization of a strong anti-idiotypic antibody of the betatype, clone 12E12, capable of mimicking SMZ, permitted the development of immunoassay formats for SMZ based on the idiotypic and anti-idiotypic approach and the direct measurement of SMZ content in diluted pig urine. The availability of the strong betatypic anti-idiotypic antibody acting as a surrogate ligand for SMZ enables antibodies to be labeled instead of ligands and offers interesting possibilities for the development of competitive type non-isotopic immunoassays for SMZ.
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Keywords: ANTI-IDIOTYPIC; ANTIBODY; FLUORESCENCE; IMMUNOASSAY; MONOCLONAL; SULFAMETHAZINE; TIME-RESOLVED

Document Type: Research Article

Affiliations: 1: Department of Biological Regulation, Weizmann Institute of Science, Rehovoth, 76100 Israel 2: Teagasc, The National Food Centre, Dunsinea, Castleknock, Dublin 15, Ireland

Publication date: September 1, 2000

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