Alteration of the Binding Characteristics of a Recombinant scFv Anti-parathion Antibody - 2. Computer Modelling of Hapten Docking and Correlation with ELISA Binding
Recombinant antibody technology and the ability to manipulate antibody binding characteristics offers much potential to agri-food analysis, and in particular to the generation of antibodies against pesticides. As part of the development of a mutagenesis strategy aimed at changing antibody specificity, the sequence of an scFv recombinant antibody specific to ethyl-parathion has been used to generate a molecular model using ProMod software. Docking studies showed the involvement in the binding pocket of CDR H3, as expected, but also of CDR L2 and of small areas of the framework region of the light chain, based on the Kabat definition of CDRs. The majority of the L2 contacts do, however, fall within a recently proposed re-definition of L2. The validity of the model was supported by comparing docking studies of structurally related pesticides with cross-reaction data from an ELISA.
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Document Type: Research Article
Publication date: September 1, 1999