Macrorhabdus ornithogaster is an opportunistic yeast that colonizes the gastric mucosa of many avian species. Until now, no studies have focused on the influence of a gastric infection on the balance of the intestinal microbiota of birds. In this study, 44 faecal samples from
individual canaries, with and without M. ornithogaster infection, were analysed. The detection of the yeast was evaluated by 18S rRNA PCR. In order to evaluate the impact of the Macrorhabdus infection on the bacterial communities, culture-independent methods, by the use of amplicon-based
sequencing as well as 16S rRNA-DGGE, were adopted. The different health status of animals affected the relative abundance of the main OTUs, with a greater diversification of the gut microbiota in healthy animals compared to the infected. In particular, Lactococcus, Pseudomonas, Acinetobacter,
Lachnospiraceae, Propionibacterium and Weissella were found to be characteristic of uninfected animals (FDR < 0.05), while Lactobacillus and Candidatus Arthromitus were characteristic of infected animals (FDR < 0.05).
Both these taxa have been reported as immunostimulatory, involved in immunological disorders. In infected animals the inferred metagenome assessed by PICRUST clearly showed a positive correlation between the presence of M. ornithogaster and KEGG genes related to ether lipid metabolism,
already reported to be immunostimulatory by activation of macrophages and to play a pathophysiological role in several immunological disorders. Finally, our results show an interaction between infection of the digestive tract and intestinal microbiota of pet birds and provide insight into
the changing of the complex enteric bacterial community.
Macrorabdus ornithogaster is a gastric yeast that colonizes a wide range of birds.
were found between infected and healthy animals in gut microbiota.
Candidatus Arthromitus was closely associated with infected birds.
M. ornithogaster can affect intestinal microbiota composition
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16s rRNA amplicon based sequencing;
Document Type: Research Article
Department of Veterinary Sciences, University of Torino, Grugliasco, Italy
Department of Agriculture, Forestry and Food Science, University of Torino, Grugliasco, Italy
School of Biosciences and Veterinary Medicine University of Camerino, Matelica, Italy
Publication date: March 4, 2019
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