Skip to main content
padlock icon - secure page this page is secure

Changes in gut bacterial communities in canaries infected by Macrorhabdus ornithogaster

Buy Article:

$60.00 + tax (Refund Policy)

Macrorhabdus ornithogaster is an opportunistic yeast that colonizes the gastric mucosa of many avian species. Until now, no studies have focused on the influence of a gastric infection on the balance of the intestinal microbiota of birds. In this study, 44 faecal samples from individual canaries, with and without M. ornithogaster infection, were analysed. The detection of the yeast was evaluated by 18S rRNA PCR. In order to evaluate the impact of the Macrorhabdus infection on the bacterial communities, culture-independent methods, by the use of amplicon-based sequencing as well as 16S rRNA-DGGE, were adopted. The different health status of animals affected the relative abundance of the main OTUs, with a greater diversification of the gut microbiota in healthy animals compared to the infected. In particular, Lactococcus, Pseudomonas, Acinetobacter, Lachnospiraceae, Propionibacterium and Weissella were found to be characteristic of uninfected animals (FDR < 0.05), while Lactobacillus and Candidatus Arthromitus were characteristic of infected animals (FDR < 0.05). Both these taxa have been reported as immunostimulatory, involved in immunological disorders. In infected animals the inferred metagenome assessed by PICRUST clearly showed a positive correlation between the presence of M. ornithogaster and KEGG genes related to ether lipid metabolism, already reported to be immunostimulatory by activation of macrophages and to play a pathophysiological role in several immunological disorders. Finally, our results show an interaction between infection of the digestive tract and intestinal microbiota of pet birds and provide insight into the changing of the complex enteric bacterial community.

HIGHLIGHTS

Macrorabdus ornithogaster is a gastric yeast that colonizes a wide range of birds.


Differences were found between infected and healthy animals in gut microbiota.


Candidatus Arthromitus was closely associated with infected birds.


M. ornithogaster can affect intestinal microbiota composition of canaries.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: 16s rRNA amplicon based sequencing; 16s rRNA-DGGE; Macrorhabdus ornithogaster; Serinus canaria; gastric yeast; gut microbiota

Document Type: Research Article

Affiliations: 1: Department of Veterinary Sciences, University of Torino, Grugliasco, Italy 2: Department of Agriculture, Forestry and Food Science, University of Torino, Grugliasco, Italy 3: School of Biosciences and Veterinary Medicine University of Camerino, Matelica, Italy

Publication date: March 4, 2019

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more