Avian reovirus-induced apoptosis related to tissue injury
Apoptosis plays an important role in pathogenesis of many viral infections. Infection of chicken with avian reovirus S1133 causes tissue injury related to virus-induced apoptosis. To determine whether avian reovirus (ARV) induced apoptosis in chicken tissues, six 3-week-old specific pathogen free White Leghorn chicks were inoculated with ARV S1133. Tissues were dual-labelled for the simultaneous detection of viral antigen containing and apoptotic cells. DNA laddering was detected in ARV-infected but not mock-infected chicken tissues. Dual-labelling assay revealed that the majority of antigen-expressing cells were not apoptotic. Surprisingly, some apoptotic but non-antigen-expressing cells were frequently located in the vicinity of antigen-expressing cells. Syncytium formation in ARV-infected chicken tissues undergoing apoptosis was apparent, suggesting a correlation between virus replication and apoptosis in chicken tissues.
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Document Type: Research Article
Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan,Graduate Institute of Biotechnology, National Pingtung University of Science and Technology, Pingtung, Taiwan
Department of Life Science, Tzu-Chi University, Hualien, Taiwan
Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan
Publication date: April 1, 2007
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