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Open Access Protective effect of two Mycoplasma gallisepticum protein fractions affinity purified with monoclonal antibodies

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Two protein fractions of Mycoplasma gallisepticum (Mg) were affinity purified with monoclonal antibodies A3 and B3, and tested for protective capacity in chickens. One fraction, designated MgP1, appeared as a doublet of 64 and 62kDa bands in sodium dodecyl sulphate-polyacrylamide gel electrophoresis gels, while MgP2 consisted of five polypeptides (64, 56, 47, 45 and 43 kDa). The molecular mass, haemagglutination activity and matching amino acid sequence of MgP1 suggest that it is identical to pMGA1.2, the putative haemagglutinin of Mg. Groups of Mg-free chickens were immunized once or twice with 1 or 5 g MgP1 or MgP2, or a combination of the two, and adjuvanted with immunostimulating complexes. Except for the group given 1 g MgP1, all vaccinated groups showed a significant (P < 0.01) reduction in air sac lesions after challenge compared with unvaccinated controls. MgP2 appeared more protective than MgP1. Vaccination twice with MgP2 was the only regime that produced a detectable serum antibody response.

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Document Type: Review Article

Affiliations: 1: Department of Poultry, National Veterinary Institute, PO Box 7073, SE– 75007 Uppsala, Sweden 2: Department of ISCOM Technology, National Veterinary Institute, PO Box 7073, SE– 75007 Uppsala, Sweden 3: Ludwig Institute for Cancer Research, PO Box 595, SE–75124 Uppsala, Sweden 4: Veterinary Medical Research Institute, PO Box 18, H–1581 Budapest, Hungary

Publication date: August 1, 2000

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