Quantification of chicken anaemia virus by competitive polymerase chain reaction
A quantitative method for chicken anaemia virus (CAV) was developed using competitive polymerase chain reaction (PCR). Competitive template was constructed by deletion of 33 nucleotides from a wildtype DNA clone of CAV. Quantification of CAV DNA molecules by the competitive PCR was rapid and highly reproducible when compared with conventional infectivity titration methods. The ratios of the viral DNA molecules and infectivity titres in MDCC-MSB1 cells varied between 1.3 and 3.55 log10 among several isolates, suggesting the existence of different infection efficiencies to MDCC-MSB1 cells by isolates. The competitive PCR will be useful for studying CAV infection in vivo and/or in vitro.
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Document Type: Review Article
Department of Virology, National Institute of Animal Health, 3-1-1 Kannondai, Tsukuba, Ibaraki, 305-0856 Japan
Shimane Prefectural Livestock Hygiene Research Institute, 918-4, Jinzaioki, Izumo, Shimane, 699-0822 Japan
Central Veterinary Research Institute, P .O. Box 33980, Balmoral, Lusaka, Zambia
August 1, 2000
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