Skip to main content
padlock icon - secure page this page is secure

Open Access Quantification of chicken anaemia virus by competitive polymerase chain reaction

Download Article:
A quantitative method for chicken anaemia virus (CAV) was developed using competitive polymerase chain reaction (PCR). Competitive template was constructed by deletion of 33 nucleotides from a wildtype DNA clone of CAV. Quantification of CAV DNA molecules by the competitive PCR was rapid and highly reproducible when compared with conventional infectivity titration methods. The ratios of the viral DNA molecules and infectivity titres in MDCC-MSB1 cells varied between 1.3 and 3.55 log10 among several isolates, suggesting the existence of different infection efficiencies to MDCC-MSB1 cells by isolates. The competitive PCR will be useful for studying CAV infection in vivo and/or in vitro.

16 References.

No Supplementary Data.
No Article Media
No Metrics

Document Type: Review Article

Affiliations: 1: Department of Virology, National Institute of Animal Health, 3-1-1 Kannondai, Tsukuba, Ibaraki, 305-0856 Japan 2: Shimane Prefectural Livestock Hygiene Research Institute, 918-4, Jinzaioki, Izumo, Shimane, 699-0822 Japan 3: Central Veterinary Research Institute, P .O. Box 33980, Balmoral, Lusaka, Zambia

Publication date: August 1, 2000

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more