Chenopodium ambrosioides L. can tolerate high concentrations of manganese and has potential for its use in the revegetation of manganese mine tailings. Following a hydroponic investigation, transmission electron microscopy (TEM)-energy disperse spectroscopy (EDS) was used to
study microstructure changes and the possible accumulation of Mn in leaf cells of C. ambrosioides in different Mn treatments (200, 1000, 10000 μmol·L−1). At 200 μmol·L−1, the ultrastructure of C. ambrosioides was
clearly visible without any obvious damage. At 1000 μmol·L−1, the root, stem and leaf cells remained intact, and the organelles were clearly visible without any obvious damage. However, when the Mn concentration exceeded 1000 μmol·L−1
the number of mitochondria in root cells decreased and the chloroplasts in stem cells showed a decrease in grana lamellae and osmiophilic granules. Compared to controls, treatment with 1000 μmol·L−1 or 10000 μmol·L−1 Mn over 30 days,
gave rise to black agglomerations in the cells. At 10000 μmol·L−1, Mn was observed to form acicular structures in leaf cells and intercellular spaces, which may be a form of tolerance and accumulation of Mn in C. ambrosioides. This study has furthered
the understanding of Mn tolerance mechanisms in plants, and is potential for the revegetation of Mn-polluted soils.
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Chenopodium ambrosioides L;
Document Type: Research Article
School of Metallurgy and Environment, Central South University, Changsha, PR China
Crop and Environment Sciences Department, Harper Adams University, Newport, Shropshire, United Kingdom
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, PR China
Publication date: July 2, 2016