Combined use of Alkane-Degrading and Plant Growth-Promoting Bacteria Enhanced Phytoremediation of Diesel Contaminated soil
Inoculation of plants with pollutant-degrading and plant growth-promoting microorganisms is a simple strategy to enhance phytoremediation activity. The objective of this study was to determine the effect of inoculation of different bacterial strains, possessing alkane-degradation and
1-amino-cyclopropane-1-carboxylic acid (ACC) deaminase activity, on plant growth and phytoremediation activity. Carpet grass (Axonopus affinis) was planted in soil spiked with diesel (1% w/w) for 90 days and inoculated with different bacterial strains, Pseudomonas sp. ITRH25, Pantoea sp. BTRH79 and Burkholderia sp. PsJN, individually and in combination. Generally, bacterial application increased total numbers of culturable hydrocarbon-degrading bacteria in the rhizosphere of carpet grass, plant biomass production, hydrocarbon degradation and reduced
genotoxicity. Bacterial strains possessing different beneficial traits affect plant growth and phytoremediation activity in different ways. Maximum bacterial population, plant biomass production and hydrocarbon degradation were achieved when carpet grass was inoculated with a consortium of
three strains. Enhanced plant biomass production and hydrocarbon degradation were associated with increased numbers of culturable hydrocarbon-degrading bacteria in the rhizosphere of carpet grass. The present study revealed that the combined use of different bacterial strains, exhibiting different
beneficial traits, is a highly effective strategy to improve plant growth and phytoremediation activity.
Keywords: hydrocarbons; phytoremediation; plant-bacteria partnership
Document Type: Research Article
Affiliations: 1: Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, Pakistan 2: Soil and Environmental Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan
Publication date: 02 December 2014
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