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Technical Note: Degradation of Phenanthrene and Anthracene by Pseudomonas Strain, Isolated From Coastal Area

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A bacterial strain was isolated from a Mumbai coastal area. It was dosed with anthracene and phenanthrene, and, after 14 days of incubation, it had degraded 90% and 93% of the anthracene and phenanthrene, respectively. The metabolites were extracted and identified by ultraviolet (UV)-visible light absorption, high-performance liquid chromatography, mass spectrometry, and by comparing with actual compounds and data. Neutral extracts from anthracene showed four metabolites, viz 1,2-dihydroxyanthracene, 6,7-benzocoumarin, 1-methoxy-2-hydroxyanthracene, and 9,10 anthraquinone. When Pseudomonas were grown in the presence of phenanthrene, two metabolites, viz 9,10-dihydroxyphenanthrene and 3,4-dihydroxyphenanthrene were identified.
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Keywords: HPLC; Pseudomonas; UV-visible light absorption; anthracene; mass spectroscopy; phenanthrene

Document Type: Research Article

Affiliations: 1: Department of Biotechnology, Mahatma Gandhi Institute of Applied Sciences, Jaipur, India 2: Department of Zoology, University of Rajasthan, Jaipur, India

Publication date: 01 April 2008

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