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Comparison of adipose and bone marrowderived stem cells in protecting against oxLDLinduced inflammation in M1macrophagederived foam cells

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Adiposederived stem cells (ADSCs) and bone marrowderived stem cells (BMSCs) are considered to be prospective sources of mesenchymal stromal cells (MSCs), that can be used in cell therapy for atherosclerosis. The present study investigated whether ADSCs cocultured with M1 foam macrophages via treatment with oxidized lowdensity lipoprotein (oxLDL) would lead to similar or improved antiinflammatory effects compared with BMSCs. ADSCs, peripheral blood monocytes, BMSCs and oxLDL were isolated from ten coronary heart disease (CHD) patients. After three passages, the supernatants of the ADSCs and BMSCs were collected and systematically analysed by liquid chromatographyquadrupole timeofflightmass spectrometry (6530; Agilent Technologies, Inc., Santa Clara, CA, USA). Cis9, trans11 was deemed to be responsible for the potential differences in the metabolic characteristics of ADSCs and BMSCs. These peripheral blood monocytes were characterized using flow cytometry. Following peripheral blood monocytes differentiation into M1 macrophages, the formation of M1 foam macrophages was achieved through treatment with oxLDL. Overall, 2x106 ADSCs, BMSCs or BMSCs+cis9, trans11 were cocultured with M1 foam macrophages. Antiinflammatory capability, phagocytic activity, antiapoptotic capability and cell viability assays were compared among these groups. It was demonstrated that the accumulation of lipid droplets decreased following ADSCs, BMSCs or BMSCs+cis9, trans11 treatment in M1 macrophages derived from foam cells. Consistently, ADSCs exhibited great advantageous antiinflammatory capabilities, phagocytic activity, antiapoptotic capability activity and cell viability over BMSCs or BMSCs+cis9, trans11. Additionally, BMSCs+cis9, trans11 also demonstrated marked improvement in antiinflammatory capability, phagocytic activity, antiapoptotic capability activity and cell viability in comparison with BMSCs. The present results indicated that ADSCs would be more appropriate for transplantation to treat atherosclerosis than BMSCs alone or BMSCs+cis9, trans11. This may be an important mechanism to regulate macrophage immune function.
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Document Type: Research Article

Affiliations: 1: Division of Cardiovascular Surgery, Second Affiliated Hospital of Harbin Medical University, Key Laboratory of Education Ministry for Myocardial Ischemia, Harbin, Heilongjiang 150086, P.R. China 2: Division of Thoracic Surgery, Tang Du Hospital of Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China 3: Division of Epidemiology and Biostatistics, School of Public Health, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China

Publication date: January 1, 2019

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  • Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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