MicroRNA-199b targets the regulation of ZEB1 expression to inhibit cell proliferation, migration and invasion in nonsmall cell lung cancer
Lung cancer is one of the leading causes of cancer-associated mortality worldwide. Previous evidence suggested that microRNAs (miRs) exhibit important regulatory roles in tumorigenesis and tumor development, including in nonsmall cell lung cancer (NSCLC). The present study investigated the expression of miR199b in NSCLC tissues and cell lines, in addition to the biological roles of miR199b in the carcinogenesis and progression of NSCLC. The results of the present study demonstrated that miR199b expression was decreased in NSCLC tissues and cell lines compared with matched adjacent healthy tissues and a healthy human bronchial epithelial cell line, respectively. An MTT assay demonstrated that the viability of NSCLC cells was decreased by miR199b. The migratory and invasive abilities of NSCLC cells were suppressed by miR199b overexpression. In addition, zinc finger Eboxbinding homeobox 1 (ZEB1) was identified to be a novel direct downstream and functional target for miR199b in NSCLC, using bioinformatics analysis, luciferase reporter assay, the reverse transcriptionquantitative polymerase chain reaction and western blotting. ZEB1 underexpression mimicked the roles of miR199b overexpression in NSCLC cells. In conclusion, the present study demonstrated that miR199b was downregulated in NSCLC and acted as a tumor suppressor by targeting ZEB1.
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Document Type: Research Article
Affiliations: Department of Thoracic Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China
Publication date: October 1, 2017
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- Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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