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Anti-inflammatory effect of tribulusamide D isolated from Tribulus terrestris in lipopolysaccharide-stimulated RAW264.7 macrophages

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TribulusĀ terrestris (T. terrestris) has been used as a traditional medicine for the treatment of a variety of diseases, including inflammation, edema and hypertension. The aqueous and ethanol extracts of T. terrestris contain alkaloids, flavonoids, tannins, quinines and phenolic compounds. Tribulusamide D is a compound that has been isolated from the ethanol extract of T. terrestris. The present study investigated the antiinflammatory effect of tribulusamide D on lipopolysaccharide (LPS)stimulated RAW 264.7 macrophages. Tribulusamide D inhibited the production of LPSinduced nitric oxide and prostaglandin E2, by reducing the expression of inducible nitric oxide synthase and cyclooxygenase2 expression, respectively. The expression of these genes associated with inflammation was determined using reverse transcriptionpolymerase chain reaction and western blot analysis. Furthermore, tribulusamide D reduced the expression of LPSinduced inflammatory cytokines, including interleukin (IL)6, IL10 and tumor necrosis factorα. They were quantified using an enzymelinked immunosorbent assay. In addition, the present study confirmed that the inhibitory effects of tribulusamide D on the inflammatory response were mediated through inactivation of mitogenactivated protein kinase p38 and inhibition of nuclear localization of nuclear factorB, which were also determined by western blot analysis. To the best of our knowledge, the current study is the first to demonstrate that tribulusamide D exerts antiinflammatory activity by altering the expression of inflammatory mediators and cytokines, indicating that tribulusamide D could be developed as a potential therapeutic agent for the treatment of inflammatory disorders.
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Document Type: Research Article

Affiliations: 1: Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan, Chungcheongnam 31116, Republic of Korea 2: Biocenter, Gyeonggi Institute of Science & Technology Promotion, Suwon, Gyeonggi 443270, Republic of Korea

Publication date: 01 October 2017

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  • Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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