Chlorogenic acid prevents inflammatory responses in IL1βstimulated human SW1353 chondrocytes, a model for osteoarthritis
Chlorogenic acid (CGA), which is a natural compound found in various plants, has been reported to exert notable antiinflammatory activities. The present study investigated the effects and underlying mechanism of CGA on interleukin (IL)1βinduced osteoarthritis (OA) chondrocytes.
An in vitro OAlike chondrocyte model was established using IL1βstimulated human SW1353 chondrocytes. Cell viability was assessed using an MTT assay. Nitric oxide (NO) and IL6 production were evaluated by Griess reaction and ELISA, respectively. The expression levels of inducible
nitric oxide synthase (iNOS), prostaglandin E2 (PGE2), cyclooxygenase 2 (COX2), collagen II, matrix metalloproteinase (MMP)13, p65 nuclear factor (NF)κB and inhibitorκBα were detected by western blot analysis. The results indicated that CGA reversed IL1βinduced increases
in iNOS/NO, IL6, MMP13 and COX2/PGE2 production, and reversed the IL1βmediated downregulation of collagen II. In addition, the data suggested that CGA was capable of inhibiting the IL1βinduced inflammatory response, at least partially via the NFκB signaling pathway. In conclusion,
CGA may be considered a suitable candidate agent in the treatment of OA.
Document Type: Research Article
Affiliations: 1: Translational Medicine Center, HongHui Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710054, P.R. China 2: Department of Pharmacy, School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710054, P.R. China
Publication date: 01 August 2017
- Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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