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Resveratrol protects against oxidized lowdensity lipoproteininduced human umbilical vein endothelial cell apoptosis via inhibition of mitochondrialderived oxidative stress

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Resveratrol, a natural phytochemical found in grapes and red wine, has been found to possess protective effects against endothelial cell apoptosis and oxidative damage. Oxidizedlow density lipoprotein (oxLDL) can induce apoptosis of endothelial cells, which is an important initial event in several cardiovascular diseases. However, the effect of resveratrol on oxLDLinduced apoptosis and oxidative damage, and the possible associated mechanisms remain to be elucidated. In the present study, following exposure to oxLDL, human umbilical vein endothelial cells (HUVECs) were treated with or without resveratrol. Cell viability was examined using Cell Counting Kit8 and 5bromo2'deoxyuridine uptake assays, respectively. Cell apoptosis was determined by flow cytometry. Apoptosisassociated markers were detected using western blot analysis. Oxidative stress was analyzed using molecular and biochemical approaches. Resveratrol restored oxLDLinduced HUVEC injury and apoptosis in a dosedependent manner. In addition, compared with the control group, oxLDL treatment decreased the B cell lymphoma2 (Bcl2)/Bcl2associated X protein ratio, mitochondrial membrane potential and activation of superoxide dismutase, and enhanced the release of mitochondrial cytochrome c into the cytoplasm, the activation of caspase and lipid peroxidation. All these alterations were significantly inhibited following treatment with resveratrol. The results demonstrated that resveratrol prevented HUVEC apoptosis through inhibiting mitochondriaderived oxidative damage. These findings may provide a novel mechanism by which resveratrol prevents against endothelial cell apoptosis.
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Document Type: Research Article

Affiliations: 1: Department of Cardiology, Tongchuan Kuangwuju Central Hospital, Shaanxi Coal and Chemical Industry Group, Tongchuan, Shaanxi 727000, P.R. China 2: Department of General Medicine, Baoji Central Hospital, Baoji, Shaanxi 721008, P.R. China 3: Department of Anesthesiology, Sun Yatsen Memorial Hospital, Sun Yatsen University, Guangzhou, Guangdong 510120, P.R. China

Publication date: January 1, 2017

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  • Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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