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Effects of inactivated Enterococcus faecalis on the proliferation and osteogenic induction of osteoblasts

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The present study aimed to evaluate the effects of Enterococcus faecalis, inactivated by the common intracanal irrigants sodium hypochlorite (NaOCl) and chlorhexidine (CHX), on osteoblasts. E. faecalis was inactivated with 2% CHX or 5.25% NaOCl. Subsequently, the Cell Counting kit8 assay was used to examine the effects of CHX and NaOCl-inactivated E. faecalis on MC3T3E1 osteoblast cell proliferation. Alizarin red staining was used to determine osteoblast mineralization, and osteogenic induction was quantified by determining the optical density of the dye solution. The relative expression levels of osteogenic genes were detected after 1, 4, 7 and 14 days of stimulation with CHX and NaOCl-inactivated E. faecalis by reverse transcriptionquantitative polymerase chain reaction. The results indicated that CHXinactivated E. faecalis inhibited osteoblast proliferation, whereas NaOClinactivated E. faecalis did not suppress cell proliferation. Various concentrations of CHX and NaOClinactivated E. faecalis induced different degrees of osteoblast mineralization. The expression levels of osteocalcin, alkaline phosphatase, runtrelated transcription factor 2, osteopontin and osterix were upregulated in cells following stimulation with 107 and 105 colonyforming units/ml E. faecalis inactivated by CHX and NaOCl; the upregulation of these osteogenic genes occurred at various time points. In conclusion, the present study demonstrated that CHXinactivated E. faecalis exerted more of an effect on osteoblast proliferation compared with NaOClinactivated E. faecalis. In addition, CHX and NaOClinactivated E. faecalis was able to induce mineralization and relevant osteogenic gene expression in osteoblast cells.
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Document Type: Research Article

Affiliations: 1: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Sun Yatsen University, Guangzhou, Guangdong 510055, P.R. China 2: Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yatsen University, Guangzhou, Guangdong 510055, P.R. China

Publication date: January 1, 2016

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  • Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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