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Downregulation of lncRNA CASC2 by microRNA-21 increases the proliferation and migration of renal cell carcinoma cells

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Several long non-coding RNAs (lncRNAs) have been identified that may have a crucial role in tumor progression and metastasis. The lncRNA cancer susceptibility candidateĀ 2 (CASC2) has previously been reported to act as a tumor suppressor gene in glioma and colorectal cancer. However, the expression and function of CASC2 in renal cell carcinoma (RCC) remains to be elucidated. The present study confirmed that CASC2 was downregulated in human RCC tissues and human RCC cell lines (786O and A498). Restoration of CASC2 expression via transfection with a pcDNA3.1(+)CASC2 vector was able to inhibit cell proliferation and migration in 786O and A498 cells, as compared with in the cells transfected with a pcDNA3.1(+) empty vector. MicroRNA21 (miR21) has been reported to be upregulated in human RCC tissues and cell lines, and is associated with the malignant progression of RCC. In the present study, bioinformatics analysis and dualluciferase reporter assays confirmed that CASC2 was a direct target gene of miR21. miR21 was able to decrease the expression of CASC2 in 786O and A498 cells. Furthermore, overexpression of miR21 partly abrogated CASC2mediated inhibition of 786O and A498 cell proliferation and migration. The present study provides evidence indicating that CASC2 targeted by miR21 acts as a tumor suppressor in RCC. Therefore, CASC2 may be considered a novel target for the diagnosis and treatment of RCC.
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Document Type: Research Article

Affiliations: Department of Urology, The Third Affiliated Hospital of Soochow University, Changzhou, JiangsuĀ 213003, P.R. China

Publication date: July 1, 2016

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  • Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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