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MicroRNA21 regulates cell proliferation and apoptosis in H2O2stimulated rat spinal cord neurons

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Oxidative stress can alter the expression level of microRNAs (miRNAs) and has a role in oxidative damage generated by reactive oxygen species (ROS). While previous studies have demonstrated that miR146a, miR21 and miR150 are essential for ROS production in heart disease, the role of these miRNAs in spinal cord injuries has not yet been examined. The present study focused on examining the role of miR146a, miR21 and miR150 during H2O2 stimulation in rat neuronal spinal cord (RNsc) cells. RNsc cells were treated with H2O2, and cells were harvested for reverse transcription quantitative polymerase chain reaction (RTqPCR) to detect the expression levels of miR146a, miR21 and miR150. The results demonstrated that miR146a, miR21 and miR150 expression was upregulated during H2O2 treatment. T-cell death and apoptosis were investigated using an MTT assay and flow cytometric analysis, respectively. Following miR21 silencing, H2O2induced cell death and apoptosis were reduced in RNsc cells, while miR150 silencing had no effect. Furthermore, Smad7 was identified as a direct target of miR21 using a Luciferase reporter assay, RT-qPCR and western blot analysis. In addition, while H2O2 downregulated Smad7 protein expression, this was reversed by inhibiting miR21 expression. Based on previous studies, it was predicted that miR21 has a role in ROS production through regulating Smad7 in rat spinal cord neurons.
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Document Type: Research Article

Affiliations: 1: Department of Orthopedics, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510632, P.R. China 2: Department of Surgery, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510632, P.R. China 3: College of Life Science and Technology, Jinan University, Guangzhou, Guangdong 510632, P.R. China

Publication date: January 1, 2015

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  • Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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