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Screening biomarkers of bladder cancer using combined miRNA and mRNA microarray analysis

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Biomarkers, such as microRNAs (miRNAs) may be useful for the diagnosis of bladder cancer. In order to understand the molecular mechanisms underlying bladder cancer, differentially expressed miRNAs (DEmiRNAs) and their target genes in bladder cancer were analyzed. In the present study, miRNA and mRNA expression profiles (GSE40355) were obtained from the Gene Expression Omnibus. These consisted of healthy bladder samples (n=8) and urothelial carcinoma samples (lowgrade, n=8 and highgrade, n=8). DEmiRNAs and differentially expressed genes (DEGs) were identified using the limma package and the Benjamin and Hochberg method from the multtest package in R. Target genes of DEmiRNAs were screened. Associations between DEGs were investigated using STRING, and an interaction network was constructed using Cytoscape. Functional and pathway enrichment analyses were performed for DEGs from the interaction network. 87 DEmiRNAs and 2058 DEGs were screened from lowgrade bladder cancer samples, and 40 DEmiRNAs and 2477 DEGs were screened from highgrade bladder cancer samples. DEtarget genes were significantly associated with the regulation of cell apoptosis. Bladder cancer, nonsmall cell lung cancer and pancreatic cancer biological pathways were found to be enriched. The results of the present study demonstrated that E2F transcription factor 1, which is targeted by miR106b, and cyclindependent kinase inhibitor 2A (CDKN2A) and VErbB2 avian erythroblastic leukemia viral oncogene homolog2, which are targeted by miR125b, participate in the bladder cancer pathway. In conclusion, DEmiRNAs in bladder cancer tissue samples and DEtargeted genes, such as miR106b and CDKN2A, which were identified in the present study, may provide the basis for targeted therapy for breast cancer and enhance understanding of its pathogenesis.
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Document Type: Research Article

Affiliations: Department of Urology, ChinaJapan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China

Publication date: August 1, 2015

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  • Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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