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Circulating microRNA122, 21 and 223 as potential markers of liver injury following warm ischaemia and reperfusion in rats

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The liver enzymes aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) are commonly used but not specific markers to quantify hepatic injury. In this in vivo study it was determined whether hepatic expression and serum levels of the microRNAs (miRNA) miR122, 21 and 223 are altered and correlated with the release of liver enzymes after warm hepatic ischaemia and reperfusion (IR). Male Wistar rats were subjected to either 45 min of partial (70%) hepatic ischaemia and 240 min of reperfusion (n=7) or sham operation (n=5). Expression levels of miR122, 21 and 223 were analysed in serum and liver tissue by quantitative polymerase chain reaction and tested for correlation with serum activities of AST, ALT and LDH. The relative expression levels of circulating miR122 increased after IR and correlated with the serum activity of AST, ALT and LDH. Neither increased serum level of miR21 nor elevated relative hepatic expression of miR223 correlated with the serum activity of liver enzymes. The hepatic expression of miR122 was unaffected by IR. The correlation between circulating miR122 expression levels and liver enzyme activity qualifies miR122 as a potential biomarker of warm hepatic IR injury.
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Document Type: Research Article

Affiliations: 1: Department of Anaesthesiology, University Hospital Duesseldorf, Duesseldorf D40225, Germany 2: Biological and Medical Research Center, Heinrich Heine University, Duesseldorf D40225, Germany

Publication date: August 1, 2015

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  • Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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