Antiangiogenic effects of resveratrol in combination with 5fluorouracil on B16 murine melanoma cells
5-Fluorouracil (5-FU) has been used as a chemotherapeutic drug for various types of cancer, although the development of resistance remains a major limitation for its use in clinical settings. In the present study, the antiangiogenic effects of resveratrol and 5FU either alone or in combination were examined in a B16 murine melanoma model. Cotreatment using resveratrol and 5FU inhibited cell proliferation more efficiently compared with use of either drug alone and the antiproliferative effect coincided with changes in the expression levels of AMPactivated protein kinase (AMPK), cyclooxygenase2, vasodilatorstimulated phosphoprotein (VASP) and vascular endothelial growth factor (VEGF). Furthermore, cotreatment with resveratrol and 5FU reduced tumor growth compared with that in the control group and this growthinhibitory effect was associated with changes in the expression levels of AMPK, VASP and VEGF. Immunohistochemical staining for angiogenesis demonstrated that cotreatment with resveratrol and 5FU reduced the number of microvascular vessels compared with that in the control group. These results suggested that cotreatment with resveratrol and 5FU suppressed cell growth and angiogenesis in B16 murine melanoma tumors.
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Document Type: Research Article
Affiliations: Department of Biological Sciences and Biotechnology, College of Life Science and Nanotechnology, Hannam University, Yusunggu, Daejeon 305811, Republic of Korea
Publication date: August 1, 2015
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