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Exogenous Nkx2.5 or GATA4transfected rabbit bone marrow mesenchymal stem cells and myocardial cell coculture on the treatment of myocardial infarction in rabbits

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The present study aimed to investigate the effects of Nkx2.5 or GATA4 transfection with myocardial extracellular environment coculture on the transformation of bone marrow mesenchymal stem cellsĀ (BMSCs) into differentiated cardiomyocytes. Nkx2.5 or GATA4 were transfected into myocardial extracellular environment cocultured BMSCs, and then injected into the periphery of infarcted myocardium of a myocardial infarction rabbit model. The effects of these gene transfections and culture on the infarcted myocardium were observed and the results may provide an experimental basis for the efficient myocardial cell differentiation of BMSCs. The present study also suggested that these cells may provide a source and clinical basis for myocardial injury repair via stem cell transplantation. The present study examined whether Nkx2.5 or GATA4 exogenous gene transfection with myocardial cell extracellular environment coculture were able to induce the differentiation of BMSCs into cardiac cells. In addition, the effect of these transfected BMSCs on the repair of the myocardium following myocardial infarction was determined using New Zealand rabbit models. The results demonstrated that myocardial cell differentiation was significantly less effective following exogenous gene transfection of Nkx2.5 or GATA4 alone compared with that of transfection in combination with extracellular environment coculture. In addition, the results of the present study showed that exogenous gene transfection of Nkx2.5 or GATA4 into myocardial cell extracellular environment cocultured BMSCs was able to significantly enhance the ability to repair, mitigating the death of myocardial cells and activation of the myocardium in rabbits with myocardial infarction compared with those of the rabbits transplanted with untreated BMSCs. In conclusion, the exogenous Nkx2.5 and GATA4 gene transfection into myocardial extracellular environment cocultured BMSCs induced increased differentiation into myocardial cells compared with that of gene transfection alone. Furthermore, significantly enhanced reparative effects were observed in the myocardium of rabbits following treatment with Nkx2.5 or GATA4transfected myocardial cell extracellular environment cocultured BMSCs compared with those treated with untreated BMSCs.
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Document Type: Research Article

Affiliations: 1: Department of Cardiac Surgery, The Third Hospital of Hebei Medical University, Hebei, Shijiazhuang 050017, P.R. China 2: Department of Histology and Embryology, Hebei Medical University, Hebei, Shijiazhuang 050017, P.R. China

Publication date: August 1, 2015

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  • Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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