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Differences in the protein expression levels of Trx2 and Prx3 in the hippocampal CA1 region between adult and aged gerbils following transient global cerebral ischemia

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The thioredoxin (Trx) and peroxiredoxin (Prx) redox system is associated with neuronal damage and neuroprotective effects via the regulation of oxidative stress in brain ischemia. In the present study, ischemiainduced changes in the protein expression levels of Trx2 and Prx3 in the stratum pyramidale (SP) of the hippocampal CA1 region were investigated in adult and aged gerbils, subjected to 5 min of transient global cerebral ischemia, using immunohistochemistry and western blot analysis. In the adult ischemiagroup, minimal Trx2 immunoreactivity was detected in the SP 2 days after ischemiareperfusion. In the aged animals, the Trx2 immunoreactivity in the shamgroup was marginally lower compared with that in the adult shamgroup. In the aged ischemiagroup, Trx2 immunoreactivity in the SP was significantly higher 1, 2 and 4 days postischemia, compared with that in the adult ischemiagroup and, in the 5 days postischemia group, Trx2 immunoreactivity was significantly decreased in the SP. Prx3 immunoreactivity in the SP of the adult ischemiagroup was significantly decreased from 4 days after ischemiareperfusion. In the aged animals, Prx3 immunoreactivity in the shamgroup was also marginally lower compared with that in the adult shamgroup. Prx3 immunoreactivity in the aged ischemiagroup was also significantly higher 1, 2 and 4 days postischemia, compared with the adult ischemiagroup; however, the Prx3 immunoreactivity was significantly decreased 5 days postischemia. The western blot analyses revealed that the pattern of changes in the protein levels of Trx2 and Prx3 in the adult and aged hippocampal CA1 region following ischemic damage were similar to the results obtained in the immunohistochemical data. These findings indicated that cerebral ischemia lead to different protein expression levels of Trx2 and Prx3 in the hippocampal CA1 region between adult and aged gerbils, and these differences may be associated with more delayed neuronal death in the aged gerbil hippocampus following transient global cerebral ischemia.
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Document Type: Research Article

Affiliations: 1: Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan, South Chungcheong 330714, Republic of Korea 2: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 200-701, Republic of Korea 3: Department of Pediatrics, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, Chuncheon, Gangwon 200-702, Republic of Korea

Publication date: August 1, 2015

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  • Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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