Downregulation of microRNA221 decreases migration and invasion in fibroblastlike synoviocytes in rheumatoid arthritis
MicroRNAs (miRNAs/miRs) are a group of noncoding RNAs that regulate the activity of target mRNAs and cellular processes, and which have been implicated in the pathogenesis of autoimmune diseases. miR221 is one of the miRNAs that regulate cell proliferation, invasion and apoptosis in tumors. However, the role of miR221 in rheumatoid arthritis (RA) remains to be fully elucidated. Therefore, the present study was undertaken to identify the role of miR221 in RA. The expression of miR221 in serum and synovial tissues of patients with RA and healthy controls was confirmed by reverse transcription quantitative polymerase chain reaction analysis. The effects of miR221 on proinflammatory cytokines and a chemokine were assessed by ELISA. The effects of miR221 on cell apoptosis, migration and invasion in fibroblastlike synoviocytes (FLS) were also assessed in vitro. The results showed that miR221 expression in serum and synovial tissues of patients with RA was higher than that in healthy controls. Downregulation of miR221 significantly suppressed the expression of proinflammatory cytokines and the chemokine, and inhibited FLS cell migration and invasion via inhibiting vascular endothelial growth factor, matrix metalloproteinase (MMP)3 and MMP9 expression. In addition, downregulation of miR221 significantly induced cell apoptosis and decreased survivin and Xlinked inhibitor of apoptosis protein expression. These findings indicated that downregulation of miR221 inhibited the expression of proinflammatory cytokines and the chemokine, suppressed FLS cell migration and invasion, and induced cell apoptosis. Therefore, miR221 is likely to be implicated in RA pathogenesis via these mechanisms, and may be a target for the treatment of RA.
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Document Type: Research Article
Affiliations: Department of Orthopaedics, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China
Publication date: August 1, 2015
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