To investigate the protective effects of acteoside against apoptosis induced by Xray radiation in human skin fibroblasts (HSFs), the cells were divided into the following groups: Control group; Xray radiation group; acteoside group, in which the confluent cells were preincubated with
50 µg/ml acteoside for 2 h followed by radiation; and positive control group, in which the cells were preincubated with 50 µg/ml paeoniflorin followed by radiation. For the radiation, HSF cells preincubated with acteoside or paeoniflorin were exposed to Xray beams
at a doserate of 3 Gy/min (16 Gy in total). Cell viability, apoptosis and intracellular alteration of redox were monitored by MTT and flow cytometry. Compared with the radiation group, the number of cells arrested at the G0/G1 phase was significantly reduced in the acteoside and
paeoniflorin groups, respectively (P<0.05). Xray radiation induced marked apoptosis in HSF cells and acteoside reversed this effect. Compared with the radiation group, the generation of intracellular reactive oxygen species (ROS) was abrogated by preincubation with acteoside or paeoniflorin
(P<0.05). In addition, the upregulation of procaspase3 induced by radiation was reversed by acteoside or paeoniflorin. Radiation could induce upregulation of Bax and downregulation of Bcl2; however, it was reversed completely after administration of acteoside or paeoniflorin. Furthermore,
the enhanced expression of ERK and JNK induced by radiation was reversed by acteoside or paeoniflorin. Acteoside could protect the cells from Xray induced damage through enhancing the scavenging activity of ROS, decreasing the Bax/Bcl2 ratio and downregulating the activity of procaspase3,
as well as modulating the mitogenactivated protein kinase signaling pathways.
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Document Type: Research Article
Department of Pharmacy, The First Affiliated Hospital, Xinjiang Medical University, Urumqi, Xinjiang Uyghur Autonomous Region 830011, P.R. China
Department of Pharmacy, The Affiliated Tumor Hospital, Xinjiang Medical University, Urumqi, Xinjiang Uyghur Autonomous Region 830011, P.R. China
Department of Natural Medicines, School of Pharmaceutical Sciences, Xinjiang Medical University, Urumqi, Xinjiang Uyghur Autonomous Region 830011, P.R. China
Publication date: August 1, 2015
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Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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