The tumorigenesis and maintenance of a cancer cells is dependent upon the collaboration of multiple signaling pathways. Signal transducer and activator of transcription 3 (STAT3) and βcatenin are at the center of multiple cancerassociated signaling pathways; therefore, simultaneously
targeting STAT3 and βcatenin may be a potential cancer treatment, leading to induced lethality of cancer cells. In the present study, HepG2 liver cancer cells were transfected with small interfering RNA (siRNA) against βcatenin and STAT3 alone or in combination. The cell growth was
assessed using an MTT assay and the levels of cell apoptosis were detected using flow cytometry. Protein levels of caspase3, cleaved caspase3, poly(ADPribose) polymerase (PARP) and cleaved PARP were determined using western blot analysis. Following siRNA transfection, βcatenin and STAT3
protein levels decreased at 72 h. HepG2 cell growth inhibition and early apoptosis in the βcatenin and STAT3 siRNA cotransfection group were significantly greater than those in the groups transfected with βcatenin or STAT3 siRNA alone. Decreased caspase3 and PARP levels,
as well as enhanced cleavage of caspase3 and PARP were observed in the βcatenin and STAT3 cotransfection group. Simultaneous silencing of βcatenin and STAT3 using siRNAs resulted in an enhanced loss of cell viability and induction of apoptosis in HepG2 liver cancer cells, suggesting
that these genes are promising targets for the further preclinical and clinical development of anticancer therapeutic strategies, which target several cancer signaling pathways simultaneously.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
Document Type: Research Article
Department of Gastroenterology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China
Department of Gastroenterology, The First Hospital of Harbin, Harbin, Heilongjiang 150000, P.R. China
Publication date: August 1, 2015
More about this publication?
Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
- Editorial Board
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Information for Advertisers
- Terms & Conditions
- Ingenta Connect is not responsible for the content or availability of external websites