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Optimized generation of survivin-specific cytotoxic T lymphocytes against lung cancer

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Cancer immunotherapy based on dendritic cells (DCs) that target survivin is a promising strategy with potential clinical applications. However, the translation of survivinspecific cytotoxic Tlymphocyte (CTL) immunotherapy into the clinical setting has numerous challenges, including the low efficiency of the treatment. The present study aimed to improve the efficiency of the treatment, and found that treatment with interleukin 4 (IL4)/granulocyte macrophage colonystimulating factor (GMCSF) and a combination of proinflammatory cytokines significantly increased the antigenpresenting and capture abilities of DCs that expressed exogenous survivin. Furthermore, lipopolysaccharide (LPS) stimulation enhanced the DC response to subsequent Tcell signals and the extent of Tcell activation. In addition, the efficiency of survivinspecific CTLs was examined, and high cytotoxicity against survivinexpressing A549 lung cancer cells was observed. However, the cytotoxicity of CTLs was significantly reduced in A549 cells with silenced survivin expression. The present study provides a novel method to optimize the generation of survivinspecific CTLs against lung cancer cells, which may advance the translation of survivinspecific CTL immunotherapy into clinical use for the treatment of cancer.
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Document Type: Research Article

Affiliations: Department of Respiratory Medicine, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China

Publication date: August 1, 2015

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  • Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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