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Knockdown of reticulon 4C by lentivirus inhibits human colorectal cancer cell growth

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Colorectal cancer is the third most common type of cancer worldwide with high cell motility and metastatic potential. Reticulon 4C (RTN4-C) is the shortest isoform of the reticulon family protein RTN4, which may act to induce cell apoptosis and suppress tumor development. The aim of the present study was to determine the role of RTN4C in colorectal cancer, and potentially identify a novel target for antitumor therapy. To investigate the biological role of RTN4C in colorectal cancer, the expression levels of RTN4C were initially analyzed in six colorectal cancer cell lines by reverse transcriptionquantitative polymerase chain reaction and western blot analysis. In addition, lentivirusbased RNA interference was utilized to knock down RTN4C expression in RKO and DLD1 cells with low and high levels of RTN4C, respectively. The rate of proliferation decreased in RTN4C silenced RKO and DLD1 cells compared with the control, as determined using MTT and colony formation assays. Flow cytometric analysis revealed that RTN4C knockdown in RKO cells led to cell cycle arrest at the G0/G1 phase, particularly at the subG1 phase representing apoptotic cells. These results indicate that RTN4C has an important role in colorectal cancer cell growth, which may provide a potential therapeutic approach for human colorectal cancer.
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Document Type: Research Article

Affiliations: 1: Neijing Institution of the Basic Medical College, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, P.R. China 2: Department of General Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. China

Publication date: August 1, 2015

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  • Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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