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Role of asymmetric dimethylarginine in rat acute lung injury induced by acute ischemic kidney injury

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The aim of the present study was to establish a rat model of acute ischemic kidney injury by continually occluding the bilateral renal artery and renal veins, as well as to examine the role of asymmetric dimethylarginine (ADMA) in acute lung injury (ALI) induced by acute kidney injury (AKI). In total, 45 male Wistar rats were randomly divided into the control, AKI and AKI + SB203580 groups. The enzymelinked immuno­sorbent assay method was used to assess the plasma levels of ADMA in each group. The activation of p38 mitogenactivated protein kinase (MAPK) and heat shock protein 27 (HSP27) was detected using western blot analysis. Immunofluorescence analyses of filamentous actin (Factin) and globular actin (Gactin) were performed. The levels of plasma ADMA and the permeability index in the AKI group were significantly higher than those in the control and AKI + SB203580 groups. The expression of phosphorylated p38 (pp38) and pHSP27 was significantly higher in the AKI and AKI + SB203580 groups compared with the control group. The AKI group also demonstrated a higher expression of pp38 and pHSP27 than the AKI + SB203580 group. The expression of Factin was more marked in the AKI group than in the control and AKI + SB203580 groups. The F/Gactin ratio in the AKI group was also higher compared with that in the control and AKI + SB203580 groups. The results from the present study indicated that plasma ADMA levels may be a good biomarker for AKIinduced ALI. In addition, the p38 MAPK/HSP27 pathway is important in modulating the levels of ADMA.
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Document Type: Research Article

Affiliations: Department of Emergency Medicine, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China

Publication date: August 1, 2015

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  • Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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